I Am Pregnant / Third Trimester··Updated: February 27, 2026

Third Trimester Lab Tests: What They Check and Why They Matter

Third trimester lab tests explained: what doctors check, why they are important, and when results may signal a concern.

Practical steps🕒 5–8 min readEasy to follow
third trimester lab testspregnancy blood testgestational diabetes screeningGBS test
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Third Trimester Lab Tests: What They Check and Why They Matter

Third trimester lab tests are a crucial part of prenatal care. They help identify conditions that could affect you or your baby before delivery.

In most cases, these tests are routine screenings. They are performed to ensure safety, not because something is wrong.


What Are Third Trimester Lab Tests?

Third trimester lab tests include blood work, urine analysis, and infection screening performed between week 24 and birth.

They may include:

  • Complete blood count
  • Glucose tolerance test
  • Urine analysis
  • Antibody screening
  • Group B Strep test

The goal is early detection and prevention.


What Exactly Do Doctors Check?

Complete Blood Count

This test evaluates:

  • Hemoglobin levels
  • Iron stores
  • Signs of inflammation

Mild anemia is common in late pregnancy due to increased blood volume.

Glucose Tolerance Test

This third trimester lab test screens for gestational diabetes.

Process:

  1. Fasting blood sample
  2. Drinking a glucose solution
  3. Follow up blood samples

Gestational diabetes is often symptom free, which makes screening essential.

Urine Testing

Urine is checked for:

  • Protein
  • Glucose

Protein in urine may indicate preeclampsia.

Group B Strep Screening

Performed at 35 to 37 weeks.

If positive, antibiotics during labor protect the baby.


Medical Background Explained Simply

During the third trimester:

  • Blood volume increases significantly
  • Insulin resistance rises
  • Placental hormones peak

These are normal physiological changes. However, in some women, they may contribute to complications.

That is why third trimester lab tests are so important.


Practical Example

Imagine a pregnant woman whose third trimester lab tests show mild anemia.

Symptoms may include:

  • Fatigue
  • Dizziness
  • Weakness

Treatment may involve:

  • Iron supplementation
  • Dietary adjustments
  • Follow up testing

When detected early, it is easy to manage.


Possible Risks Identified by Testing

Abnormal results may reveal:

  • Gestational diabetes
  • Significant anemia
  • Preeclampsia indicators
  • Infection

Untreated conditions may increase risks during delivery.


When to Call Your Doctor Immediately

Regardless of lab results, seek care if you experience:

  • Severe headache
  • Visual changes
  • Sudden swelling
  • High blood pressure
  • Decreased fetal movement

Trust your instincts.


How to Prepare

  • Follow fasting instructions
  • Stay hydrated
  • Write down questions
  • Try not to anticipate worst case scenarios

These tests are preventive tools.


A Reassuring Perspective

Third trimester lab tests exist to protect you and your baby.

Most results are normal. If something requires attention, early action makes a significant difference.

You are not being tested because something is wrong. You are being supported.

For more practical, evidence based pregnancy guidance, explore additional in depth resources designed to help you feel confident and informed.

Frequently Asked Questions

When are third trimester lab tests done?+

Glucose screening is typically performed between 24 and 28 weeks, while GBS screening is done around 35 to 37 weeks.

What do third trimester lab tests detect?+

They evaluate blood count, blood sugar levels, urine markers, infections, and Group B Streptococcus.

Are third trimester lab tests painful?+

They involve routine blood draws and urine samples. The GBS swab may feel slightly uncomfortable but is not painful.

Sources

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